Image
HEK 293 cells were treated with Ser/Thr phosphatase inhibitor,calyclinA (100 nM) for 30 min, or Tyr phosphatase inhibitor, pervanadate (1 mM) for 30 mim, and then lyzed in SDS-PAGE loading buffer.
HEK 293 cells were treated with Ser/Thr phosphatase inhibitor,calyclinA (100 nM) for 30 min, or Tyr phosphatase inhibitor, pervanadate (1 mM) for 30 mim, and then lyzed in SDS-PAGE loading buffer.
Reference | None |
---|---|
Composition of gel | Zn2+ -Phos-tag: 25µM Polyacrylamide: 8.5%(w/v) |
Detection | WB: anti-ATF2 |